RNA

Part:BBa_K2841512:Design

Designed by: Rhys Evans, Jack Lawrence, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-13)


gRNA Cis-acting factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This cis-acting factor works with S. pyogenes dCas9


Source

Larson, M., Gilbert, L., Wang, X., Lim, W., Weissman, J. and Qi, L. (2013). CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Protocols, 8(11), pp.2180-2196.

References